Both theoretical and field research have shown that protective layer mining with pressure-relief gas extraction in protected coal seams is an effective and economical way of controlling coal and gas outbursts in underground mines. Given the influences of coal seam angles, layer spacing, pressure-relief angles, coal pillars, and other factors in the protected areas of protected seams, the protection range is often smaller than the area of protective seam mining. This problem can be solved by using a gas drainage method based on coal seam geology and the conditions of coalbed methane (CBM) occurrence during protective layer mining. If a protective layer is prone to outbursts, crossing boreholes and bedding boreholes can be used to eliminate outburst hazards. The crossing boreholes should control the upper side of the roadway contour for at least 20 m and the lower side for at least 10 m. For other coal seams, the boreholes should control both sides of the roadway contour for at least 15 m. Crossing boreholes can not only extract CBM from the protective layer but can also extract from the protected coal seam, thus leading to a larger protection range in the protective layer than that predicted by the pressure-relief boundary, as well as expanding the protection range. The actual protection range of the protected layer was determined using an index of residual gas content. The results of the study indicate that the pressure-relief angle increased from 56° to 69° in the strike direction and from 75° to 90° in the inclination direction. The protection range was approximately equal to the mining area of the working face of the protective layer, thereby extending the protection range. One of the important reasons for protection range expansion was the influence of gas drainage via crossing boreholes. The protection range extension mitigated the outburst danger over a large area of the coal seam, thus enabling high efficiency and safe exploitation.

The present study screened potential genes related to lung adenocarcinoma, with the aim of further understanding disease pathogenesis. The GSE2514 dataset including 20 lung adenocarcinoma and 19 adjacent normal tissue samples from 10 patients with lung adenocarcinoma aged 45-73 years was downloaded from Gene Expression Omnibus. Differentially expressed genes (DEGs) between the two groups were screened using the t-test. Potential gene functions were predicted using functional and pathway enrichment analysis, and protein-protein interaction (PPI) networks obtained from the STRING database were constructed with Cytoscape. Module analysis of PPI networks was performed through MCODE in Cytoscape. In total, 535 upregulated and 465 downregulated DEGs were identified. These included ATP5D, UQCRC2, UQCR11 and genes encoding nicotinamide adenine dinucleotide (NADH), which are mainly associated with mitochondrial ATP synthesis coupled electron transport, and which were enriched in the oxidative phosphorylation pathway. Other DEGs were associated with DNA replication (PRIM1, MCM3, and RNASEH2A), cell surface receptor-linked signal transduction and the enzyme-linked receptor protein signaling pathway (MAPK1, STAT3, RAF1, and JAK1), and regulation of the cytoskeleton and phosphatidylinositol signaling system (PIP5K1B, PIP5K1C, and PIP4K2B). Our findings suggest that DEGs encoding subunits of NADH, PRIM1, MCM3, MAPK1, STAT3, RAF1, and JAK1 might be associated with the development of lung adenocarcinoma.

ABSTRACT Patients with systemic lupus erythematosus (SLE) have an increased susceptibility to bacterial, viral, fungal and parasitic infections. Cryptococcal infection of the central nervous system (CNS) is a rare but often fatal complication of SLE. Here, we describe a case of cryptococcal meningitis in a female patient with active SLE, who was successfully treated with amphotericin B. This case suggests that the clinical findings of SLE patients with cryptococcal meningitis are non-specific and misleading, and early use of amphotericin B has a good response.

RESUMEN Los pacientes con lupus eritematoso sistémico (LES) tienen una mayor susceptibilidad a las infecciones bacterianas, virales, fúngicas y parasitarias. La infección criptocócica del sistema nervioso central (SNC) es una complicación rara, pero a menudo fatal de LES. Aquí, describimos un caso de meningitis criptocócica en un paciente femenino con LES activo, que fue tratada con éxito con anfotericina B. Este caso sugiere que los resultados clínicos de pacientes de LES con meningitis criptocócica son no específicos y engañosos, y el uso temprano de la anfotericina B tiene una buena respuesta.

The distal cytoplasmic motifs of leukemia inhibitory factor receptor α-chain (LIFRα-CT3) can independently induce intracellular myeloid differentiation in acute myeloid leukemia (AML) cells by gene transfection; however, there are significant limitations in the potential clinical use of these motifs due to liposome-derived genetic modifications. To produce a potentially therapeutic LIFRα-CT3 with cell-permeable activity, we constructed a eukaryotic expression pcDNA3.0-TAT-CT3-cMyc plasmid with a signal peptide (ss) inserted into the N-terminal that codes for an ss-TAT-CT3-cMyc fusion protein. The stable transfection of Chinese hamster ovary (CHO) cells via this vector and subsequent selection by Geneticin resulted in cell lines that express and secrete TAT-CT3-cMyc. The spent medium of pcDNA3.0-TAT-CT3-cMyc-transfected CHO cells could be purified using a cMyc-epitope-tag agarose affinity chromatography column and could be detected via SDS-PAGE, with antibodies against cMyc-tag. The direct administration of TAT-CT3-cMyc to HL-60 cell culture media caused the enrichment of CT3-cMyc in the cytoplasm and nucleus within 30 min and led to a significant reduction of viable cells (P < 0.05) 8 h after exposure. The advantages of using this mammalian expression system include the ease of generating TAT fusion proteins that are adequately transcripted and the potential for a sustained production of such proteins in vitro for future AML therapy.